High-Resolution Wide-Field Fluorescence Micrograph of Mammalian Cell Nuclei Stained with DAPI

Create a High-resolution wide-field fluorescence micrograph of ~70 mammalian cell nuclei stained with DAPI. Non-periodic distribution with clusters and empty spaces. Nuclei vary 8–14 µm; shapes round, oval, or mildly bean-shaped. Diffuse intranuclear chromatin texture with only occasional bright foci; crisp nuclear envelopes. Pure black background with subtle shot noise and gentle vignetting from a 40×/1.3 NA oil objective. Monochrome grayscale rendering of the blue channel. Scientific realism, no cytoplasm, no scale bar, no text, no pattern repetition. 2048 × 2048 px. Negative prompt: grid pattern, seamless tiling, uniform polka-dot spots, swirling background, marble noise, halo glow, cartoon, multicolour fluorescence, watermark, text. Sampler tips • CFG ≈ 5.5 – 6 • 30–40 steps, Euler a or DPM++ 2M • Generate at 512 × 512 (or 768 × 768) and downscale or crop to 256 × 256 to sharpen details, High-resolution wide-field fluorescence micrograph displaying mammalian cell nuclei stained with DAPI. Non-periodic distribution with clusters and empty spaces. Nuclei are round, oval, or mildly bean-shaped, measuring approximately 8 to 14 µm. Background is pure black with subtle shot noise and gentle vignetting. Crisp nuclear envelopes are visible with diffuse chromatin texture and occasional bright foci. Monochrome grayscale rendering of the blue channel

Generate a high-resolution wide-field fluorescence micrograph of approximately 70 mammalian cell nuclei, stained with DAPI, and distributed non-periodically with clusters and empty spaces. The nuclei vary between 8–14 µm in size and exhibit round, oval, or mildly bean-shaped structures. The chromatin texture inside the nuclei appears diffuse with only occasional bright foci, having crisp nuclear envelopes visible. The composition should utilize a pure black background, feature subtle shot noise, and include gentle vignetting effects originating from a 40×/1.3 NA oil objective lens. The image is rendered monochrome using the grayscale values of the DAPI-stained blue channel, maintaining scientific realism without visual elements such as cytoplasm, scale bars, or text annotations. Ensure visual accuracy by avoiding grid patterns, seamless tiling, uniform polka-dot arrangements, swirling backgrounds, marble noise, halo glows, cartoon representations, or multicolor fluorescence. The image should be set to 2048 × 2048 px resolution, High-resolution fluorescence micrograph of cell nuclei with varying sizes and shapes. Non-periodic distribution with clusters among empty spaces. Nuclei sizes 8–14 µm; shapes include round and oval. Clear nuclear envelopes; diffuse chromatin texture. Black background with slight noise